Biotechnology. Requirements for evaluating the performance of quantification methods for nucleic acid target sequences. qPCR and dPCR Ingestion-build-47182 technical reports and guidelines

technical reports and guidelines

BS EN 60085 is applicable where the thermal factor is the dominant ageing factor

mechanical and geometrical characteristics of the specified pipes and fittings

The ISO 3601 series of standards basically addresses O-rings with moulded cross-sections without a radial joint

Biotechnology. Requirements for evaluating the performance of quantification methods for nucleic acid target sequences. qPCR and dPCR Ingestion-build-47182 technical reports and guidelines1 Scope This document provides generic requirements for evaluating the performance and ensuring the quality of methods used for the quantification of specific nucleic acid sequences (targets). This document is applicable to the quantification of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) target sequences using either digital (dPCR) or quantitative real time PCR (qPCR) amplification technologies. It applies to target sequences present in